Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected cytoplasmic dynein heavy chain in HeLa cells.

Research CategoryCell Structure

Research Sub CategoryCytoskeletal Signaling

This Anti-Dynein Antibody, HC, cytosolic is validated for use in Western Blotting, Immunocytochemistry for the detection of Dynein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cytoplasmic dynein 1 heavy chain 1 (UniProt Q14204; also known as Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic) is encoded by the DYNC1H1 (also known as CMT2O, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325, SMALED1) gene (Gene ID 1778) in human. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ~1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.

Immunogen

Epitope: Second AAA domain.

KLH-conjugated linear peptide corresponding to a sequence within the second AAA domain of human cytoplasmic dynein heavy chain.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected cytoplasmic dynein heavy chain in 10 µg of HeLa cell lysate.

Specificity

This polyclonal antibody targets a sequence within the second AAA domain of cytoplasmic dynein heavy chain encoded by the DYNC1H1 gene.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~500 kDa observed. 532.4 kDa calculated.